SEGH Articles

# The Joy's of PhD research in two countries

06 October 2014
Edwards project aim is to improve the accuracy and spatial resolution of dietary mineral supply estimates in Malawi and to investigate the potential of agricultural solutions to mitigate dietary mineral deficiencies. He has been conducting fieldwork in Malawi during 2012-14.

Humans require 22 mineral elements for their wellbeing including calcium (Ca), iron (Fe), magnesium (Mg), selenium (Se) and zinc (Zn). The biological functions of elements include bone structure (Ca and Mg) and, as constituents of proteins, immune response (Se and Zn) and oxygen transport (Fe and Zn). Adequate quantities of these elements in diets is thus necessary for food security; inadequate intakes, or poor absorption in the gut due to e.g. diarrhoea, can lead to ‘hidden hunger’. It’s termed ‘hidden’ because the physical effects are not obvious, unlike the symptoms of protein or energy undernourishment, and because it is often hard to quantify the prevalence of such malnourishment in populations.

Malawi hasn’t witnessed widespread famine since 2005, although certain regions are prone to food shortages due to both drought and flooding. But it may be that hidden hunger is widespread and that deficiencies of certain minerals and vitamins are a major health burden. There is evidence to suggest this is the case, for example with Se deficiency (Gibson 2011; Eick 2009; Hurst 2013) and Zn deficiency (Gibson 1998; Siyame 2013); but there is no data at the national level.

Malawi is predominantly a subsistence economy in which households grow their own food. The dietary supply of elements is thus dependent on which crops households choose to grow and what those crops contain. Element concentrations in crops depend on the availability of elements in the soil: for example, in low-pH soils Se is predominantly found in forms unavailable for plant uptake, whereas in soils with pH >6.5 Se is generally soluble, mobile and readily available for plant uptake.  There are limited data on crop composition in Malawi so we worked to fill this gap, collecting over 600 crop samples representing 97 food items for multi-element analysis by ICP-MS (Joy et al. submitted). We found that soil type affects crop composition, with maize and leafy vegetables from calcareous soils having greater Ca, Cu, Fe, Mg and Se concentrations than those grown on non-calcareous soils. Maize also had greater Zn in samples from calcareous soils, whereas leafy vegetables had greater Zn from non-calcareous soils.

To find out what crops households are growing and what foods they are eating, the Malawi Household Survey (World Bank and Malawi Government) has proved a valuable resource. In this survey, >12,500 households were asked what foods they consumed over the last seven days. We are working to match this data to our composition data to generate dietary mineral supply estimates by region and soil type. One of our early findings is the critical importance of small fish in meeting Ca, Se and Zn requirements. Most fish production is from Lake Malawi, a Rift Valley lake that runs much of the length of the country. It’s sometimes known as the Calendar Lake as it’s roughly 365 mile long and 52 miles wide. Fish are sundried before traders take them inland. Although fish is a vital source of minerals in the diet, households require some cash to purchase them. It will be very interesting to see the relationship between household income and consumption of fish.

Dried fish at a market in the capital city, Lilongwe

The FAO and World Bank have just published a book called ‘Analyzing Food Security Using Household Survey Data’ (http://www.fao.org/economic/ess/ess-fs/fs-methods/householdsurvey/en/#.U7V6cPldVPo) and at the recent Micronutrient Forum held in Addis Ababa, Ethiopia, I found that other research groups including IFPRI are doing similar work to us. It’s great to see the hard work now contributing to scientific knowledge as we write up, publish and present our findings.

Edward presenting the team’s work at Mzuzu University in northern Malawi

The last couple of years have been a steep learning curve: a faulty radiator can write off your engine; laptop battery life is everything; and you may need to hire guards to keep monkeys off your maize trials! These have been valuable lessons as I plan to continue similar research in Ethiopia starting in September. Overall it has been a pleasure living in Malawi, a country endowed with some very beautiful landscapes and incredibly friendly folk – it’s not uncommon on sampling trips to be invited into farmers’ houses for roast pumpkin and peanuts. And the Malawi Ministries of Agriculture and Health have been very supportive of the work. Hopefully the outputs will be useful to them, for example in developing fertiliser policies and targeting nutrition strategies.

by Edward Joy, PhD researcher University of Nottingham-BGS

References

Eick F, Maleta K, Govasmark E, Duttaroy AK, Bjune AG (2009) Food intake of selenium and sulphur amino acids in tuberculosis patients and healthy adults in Malawi. Int J Tuberc Lung Dis 13: 1313–1315

Gibson RS, Bailey KB, Ampong Romano AB, Thomson CD (2011) Plasma selenium concentrations in pregnant women in two countries with contrasting soil selenium levels. J Trace Elem Med Bio 25:230–235

Gibson RS, Huddle JM (1998) Suboptimal zinc status in pregnant Malawian women: its association with low intakes of poorly available zinc, frequent reproductive cycling, and malaria. Am J Clin Nutr 67:702–709

Hurst R, Siyame EWP, Young SD, Chilimba ADC, Joy EJM, Black CR, Ander EL, Watts MJ, Chilima B, Gondwe J, Kang’ombe D, Stein AJ, Fairweather-Tait SJ, Gibson RS, Kalimbira AA, Broadley MR (2013) Soil-type influences human selenium status and underlies widespread selenium deficiency risks in Malawi. Sci Rep 3: 1425. DOI: 10.1038/srep01425

Siyame EWP, Hurst R, Wawer AA, Young SD, Broadley MR, Chilimba ADC, Ander EL, Watts MJ, Chilima B, Gondwe J, Kang'ombe D, Kalimbira A, Fairweather-Tait SJ, Bailey KB, Gibson RS (2013) A high prevalence of zinc- but not iron-deficiency among women in rural Malawi: a cross-sectional study. Int J Vitam Nutr Res 83: 176–187

Joy, EJM, Broadley, MR, Young, SD, Black CR, Chilimba, ADC, Ander, EL, Barlow, TS and Watts, MJ*. (2014). A spatially refined food composition table for Malawi, Science Total Environment, (in press)

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## Science in theNews

Latest on-line papers from the SEGH journal: Environmental Geochemistry and Health

• Fertilizer usage and cadmium in soils, crops and food 2018-06-23

### Abstract

Phosphate fertilizers were first implicated by Schroeder and Balassa (Science 140(3568):819–820, 1963) for increasing the Cd concentration in cultivated soils and crops. This suggestion has become a part of the accepted paradigm on soil toxicity. Consequently, stringent fertilizer control programs to monitor Cd have been launched. Attempts to link Cd toxicity and fertilizers to chronic diseases, sometimes with good evidence, but mostly on less certain data are frequent. A re-assessment of this “accepted” paradigm is timely, given the larger body of data available today. The data show that both the input and output of Cd per hectare from fertilizers are negligibly small compared to the total amount of Cd/hectare usually present in the soil itself. Calculations based on current agricultural practices are used to show that it will take centuries to double the ambient soil Cd level, even after neglecting leaching and other removal effects. The concern of long-term agriculture should be the depletion of available phosphate fertilizers, rather than the negligible contamination of the soil by trace metals from fertilizer inputs. This conclusion is confirmed by showing that the claimed correlations between fertilizer input and Cd accumulation in crops are not robust. Alternative scenarios that explain the data are presented. Thus, soil acidulation on fertilizer loading and the effect of Mg, Zn and F ions contained in fertilizers are considered using recent $$\hbox {Cd}^{2+}$$ , $$\hbox {Mg}^{2+}$$ and $$\hbox {F}^-$$ ion-association theories. The protective role of ions like Zn, Se, Fe is emphasized, and the question of Cd toxicity in the presence of other ions is considered. These help to clarify difficulties in the standard point of view. This analysis does not modify the accepted views on Cd contamination by airborne delivery, smoking, and industrial activity, or algal blooms caused by phosphates.

• Effects of conversion of mangroves into gei wai ponds on accumulation, speciation and risk of heavy metals in intertidal sediments 2018-06-23

### Abstract

Mangroves are often converted into gei wai ponds for aquaculture, but how such conversion affects the accumulation and behavior of heavy metals in sediments is not clear. The present study aims to quantify the concentration and speciation of heavy metals in sediments in different habitats, including gei wai pond, mangrove marsh dominated by Avicennia marina and bare mudflat, in a mangrove nature reserve in South China. The results showed that gei wai pond acidified the sediment and reduced its electronic conductivity and total organic carbon (TOC) when compared to A. marina marsh and mudflat. The concentrations of Cd, Cu, Zn and Pb at all sediment depths in gei wai pond were lower than the other habitats, indicating gei wai pond reduced the fertility and the ability to retain heavy metals in sediment. Gei wai pond sediment also had a lower heavy metal pollution problem according to multiple evaluation methods, including potential ecological risk coefficient, potential ecological risk index, geo-accumulation index, mean PEL quotients, pollution load index, mean ERM quotients and total toxic unit. Heavy metal speciation analysis showed that gei wai pond increased the transfer of the immobilized fraction of Cd and Cr to the mobilized one. According to the acid-volatile sulfide (AVS) and simultaneously extracted metals (SEM) analysis, the conversion of mangroves into gei wai pond reduced values of ([SEM] − [AVS])/f oc , and the role of TOC in alleviating heavy metal toxicity in sediment. This study demonstrated the conversion of mangrove marsh into gei wai pond not only reduced the ecological purification capacity on heavy metal contamination, but also enhanced the transfer of heavy metals from gei wai pond sediment to nearby habitats.

• Cytotoxicity induced by the mixture components of nickel and poly aromatic hydrocarbons 2018-06-22

### Abstract

Although particulate matter (PM) is composed of various chemicals, investigations regarding the toxicity that results from mixing the substances in PM are insufficient. In this study, the effects of low levels of three PAHs (benz[a]anthracene, benzo[a]pyrene, and dibenz[a,h]anthracene) on Ni toxicity were investigated to assess the combined effect of Ni–PAHs on the environment. We compared the difference in cell mortality and total glutathione (tGSH) reduction between single Ni and Ni–PAHs co-exposure using A549 (human alveolar carcinoma). In addition, we measured the change in Ni solubility in chloroform that was triggered by PAHs to confirm the existence of cation–π interactions between Ni and PAHs. In the single Ni exposure, the dose–response curve of cell mortality and tGSH reduction were very similar, indicating that cell death was mediated by the oxidative stress. However, 10 μM PAHs induced a depleted tGSH reduction compared to single Ni without a change in cell mortality. The solubility of Ni in chloroform was greatly enhanced by the addition of benz[a]anthracene, which demonstrates the cation–π interactions between Ni and PAHs. Ni–PAH complexes can change the toxicity mechanisms of Ni from oxidative stress to others due to the reduction of Ni2+ bioavailability and the accumulation of Ni–PAH complexes on cell membranes. The abundant PAHs contained in PM have strong potential to interact with metals, which can affect the toxicity of the metal. Therefore, the mixture toxicity and interactions between diverse metals and PAHs in PM should be investigated in the future.